Activated macrophages induce hepcidin expression in HuH7 hepatoma cells

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Activated macrophages induce hepcidin expression in HuH7 hepatoma cells.

BACKGROUND Hepcidin is an iron regulatory peptide produced by the liver in response to inflammation and elevated systemic iron. Recent studies suggest that circulating monocytes and resident liver macrophages--Küpffer cells--may influence both basal and inflammatory expression of hepcidin. DESIGN AND METHODS We used an in vitro co-culture model to investigate hepatocyte hepcidin regulation in...

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Hypoxia inhibits hepcidin expression in HuH7 hepatoma cells via decreased SMAD4 signaling.

Hepcidin negatively regulates systemic iron homeostasis in response to inflammation and elevated serum iron. Conversely, hepcidin expression is diminished in response to hypoxia, oxidative stress, and increased erythropoietic demand, though the molecular intermediates involved are incompletely understood. To address this, we have investigated hypoxic hepcidin regulation in HuH7 hepatoma cells e...

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HCV Core Protein Uses Multiple Mechanisms to Induce Oxidative Stress in Human Hepatoma Huh7 Cells

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CYP3A4 catalytic activity is induced in confluent Huh7 hepatoma cells.

Drug-induced hepatotoxicity is an important cause for disapproval, limitations of use, or withdrawal of drugs, and there is a high need for reproducible in vitro systems that can predict such toxicity. In this study, we show that confluent growth of the human hepatoma cell line Huh7 up to 5 weeks results in increased gene expression of several cytochromes P450 (P450s), UDP-glucuronosyltransfera...

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ژورنال

عنوان ژورنال: Haematologica

سال: 2009

ISSN: 0390-6078,1592-8721

DOI: 10.3324/haematol.2008.003400